| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Griffith, Claire E (12)
|
VACCA |
27.87 |
27.33 |
-0.54 |
| 2 |
Pfaff, Alison J (11)
|
VASTRM |
27.40 |
27.56 |
0.16 |
| 3 |
Mendez-Guzman, Doreli N (12)
|
VAGATR |
28.69 |
28.31 |
-0.38 |
| 4 |
Skinner, Samantha R (12)
|
VAHOKI |
28.34 |
28.79 |
0.45 |
| 5 |
Baxley, Meagan A (12)
|
VACCA |
27.34 |
29.23 |
1.89 |
| 6 |
Dunham, Sarah G (11)
|
VAGATR |
30.73 |
29.97 |
-0.76 |
| 7 |
MacDermott, Aine (12)
|
VASTRM |
30.52 |
30.53 |
0.01 |
| 8 |
Mayock-Bradley, Susanne H (12)
|
VASTRM |
30.29 |
31.17 |
0.88 |
| 9 |
Gorby, Amelia G (11)
|
VAGATR |
32.22 |
32.23 |
0.01 |
| 10 |
Parker, Julia P (12)
|
VACCA |
32.08 |
33.29 |
1.21 |
| 11 |
Dixon, Julia * (12)
|
VAACST |
|
33.44 |
- |
| 12 |
Arnold, Michelle M (12)
|
VAGATR |
33.80 |
34.05 |
0.25 |
| 13 |
Sandridge, Desirae R (12)
|
VAGATR |
34.48 |
36.46 |
1.98 |
| 14 |
Malone, Jane E (12)
|
VAACST |
|
36.96 |
- |
| 15 |
Lambert, Maria L (12)
|
VASTRM |
|
39.11 |
- |
| 16 |
Madan, Shreya (11)
|
VACCA |
36.91 |
39.34 |
2.43 |