| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Lee, Ella K (10)
|
GUSWIM |
32.98 |
33.69 |
0.71 |
| 1 |
Vitulli, Addison G (8)
|
GUESA |
|
41.19 |
- |
| 2 |
Ferrero D'Ambrosio, Dana (10)
|
GUUN-T |
39.20 |
35.71 |
-3.49 |
| 3 |
Tippy, Marin A (10)
|
GUESA |
36.55 |
35.98 |
-0.57 |
| 4 |
Dailey, Alexis E (10)
|
GUSST |
|
39.05 |
- |
| 5 |
Gresens, Caroline E (9)
|
GUSWIM |
40.62 |
39.32 |
-1.30 |
| 6 |
Bui, Isabelle U (10)
|
GULIFE |
44.33 |
40.04 |
-4.29 |
| 7 |
Cirillo, Abbrianna L (9)
|
GUSST |
52.84 |
40.30 |
-12.54 |
| 8 |
Glowacki, Madelyn E (9)
|
GUESA |
47.72 |
46.25 |
-1.47 |
| 9 |
Gonzalez, Erika R (10)
|
GUESA |
|
46.77 |
- |
| 10 |
McCallister, Harper B (10)
|
GUTYES |
49.85 |
47.84 |
-2.01 |
| 11 |
Foster, Victoria M (10)
|
GUSWIM |
|
53.80 |
- |
| 12 |
Jones, Grace (10)
|
GULIFE |
|
55.28 |
- |
| 13 |
Gonzalez, Hope (9)
|
GULIFE |
|
57.16 |
- |
| 14 |
Blackmon, Kyra L (9)
|
GUAMBU |
55.94 |
1:00.89 |
4.95 |
| 15 |
Walton, Avery L (9)
|
GUSWIM |
|
1:03.52 |
- |
|
Medlin, Mia G (8)
|
GUESA |
46.41 |
DQ |
- |
|
Thomas, Isabella L (9)
|
GUAMBU |
|
DQ |
- |
|
Stidham, Brooklyn E (8)
|
GUAMBU |
|
DQ |
- |
|
Tipton, Kora L (9)
|
GUSST |
|
DQ |
- |
|
Lewis, Peyton A (10)
|
GUAMBU |
|
DQ |
- |
|
Lavespere, Victoria L (9)
|
GUAMBU |
|
DQ |
- |
|
Jordan, Julie (9)
|
GUAMBU |
|
DQ |
- |