| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Pierce, Zoie C (12)
|
GULIFE |
37.27 |
35.01 |
-2.26 |
| 2 |
Foit, Rachael E (12)
|
GUTEST |
37.24 |
36.05 |
-1.19 |
| 3 |
Kungu, Hope N (12)
|
GUTEST |
39.15 |
36.55 |
-2.60 |
| 4 |
Telthorster, Christie P (12)
|
GUMARC |
39.13 |
38.37 |
-0.76 |
| 5 |
Melancon, Sophie E (12)
|
GURICE |
39.49 |
38.48 |
-1.01 |
| 6 |
Cash, Audrey S (11)
|
GUPEAK |
41.01 |
39.36 |
-1.65 |
| 7 |
Lorber, Lyla G (11)
|
GUESCA |
41.52 |
40.21 |
-1.31 |
| 8 |
Mudaliar, Megna N (11)
|
GUPEAK |
44.01 |
40.61 |
-3.40 |
| 9 |
Ochsner, Nyah (11)
|
GULIFE |
|
40.99 |
- |
| 10 |
Vose, Haylee E (12)
|
GUAMBU |
42.20 |
41.12 |
-1.08 |
| 11 |
Brown, Allison A (11)
|
GUPEAK |
42.83 |
41.23 |
-1.60 |
| 12 |
Evans, Ella L (11)
|
GUTEST |
54.60 |
44.08 |
-10.52 |
| 13 |
Shen, Heyi (Fiona) H (11)
|
GUPEAK |
54.04 |
48.07 |
-5.97 |
| 14 |
Detert, Sophia J (11)
|
GUPEAK |
|
51.67 |
- |
| 15 |
Schulz, Cori K (12)
|
GUCFSC |
1:03.27 |
1:06.68 |
3.41 |
|
Hansen, Lauren E (11)
|
GUAMBU |
|
DQ |
- |