| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Finanger, Elin J (6)
|
GUTWST |
24.00 |
24.70 |
0.70 |
| 2 |
Koski, Sophia E (6)
|
GUSSAN |
|
26.52 |
- |
| 3 |
Rosebush, Emilee A (6)
|
GUMAC |
29.94 |
27.09 |
-2.85 |
| 4 |
Cooper, Bexley K (6)
|
GUSSAN |
30.39 |
27.68 |
-2.71 |
| 5 |
Rogojina, Kaitlyn G (6)
|
GUCFSC |
30.02 |
29.05 |
-0.97 |
| 6 |
Rojas, Allison J (6)
|
GUTWST |
34.28 |
31.61 |
-2.67 |
| 7 |
Roberts, Annalise C (6)
|
GUCFSC |
|
34.58 |
- |
| 8 |
Rosas Magos, Ines (6)
|
GUTWST |
1:06.89 |
43.55 |
-23.34 |
|
McCarthy, Gabrielle R (6)
|
GUCFSC |
|
DQ |
- |
|
Pope, Kenzie R (6)
|
GUTWST |
32.66 |
DQ |
- |
|
Gonzalez-Meyer, Valentina N (6)
|
GUTWST |
|
NS |
- |