| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Lopez Portillo, Regina (12)
|
GUMAC |
43.74 |
37.15 |
-6.59 |
| 2 |
Miller, Brooke C (11)
|
GUSSAN |
37.06 |
37.44 |
0.38 |
| 3 |
Lopez, Jocelyne (11)
|
GUAMBU |
39.06 |
37.51 |
-1.55 |
| 4 |
Winslow, Kate E (11)
|
GUMAC |
39.79 |
38.69 |
-1.10 |
| 5 |
Marsh, Vanessa A (11)
|
GUSHRK |
40.42 |
39.91 |
-0.51 |
| 6 |
Foote, Brooklyn S (11)
|
GUPACE |
45.17 |
41.75 |
-3.42 |
| 7 |
Diamond, Addison K (11)
|
GUKATY |
41.23 |
42.28 |
1.05 |
| 8 |
Masch, Mia G (11)
|
GUKATY |
44.05 |
42.82 |
-1.23 |
| 9 |
Pinkoski, Pallas N (11)
|
GUSHRK |
43.07 |
43.59 |
0.52 |
| 10 |
Peden, Ashlee C (12)
|
GUKATY |
41.83 |
44.94 |
3.11 |
| 11 |
Botens, Ansley E (12)
|
GUKATY |
44.06 |
45.18 |
1.12 |
| 12 |
Drewery, Laura E (11)
|
GUAMBU |
44.19 |
45.77 |
1.58 |
| 13 |
Vasquez, Mary C (12)
|
GUPACK |
42.87 |
46.21 |
3.34 |
| 14 |
Vose, Haylee E (12)
|
GUAMBU |
44.11 |
46.61 |
2.50 |
| 15 |
Richardson, Evelyn J (11)
|
GUPACK |
41.24 |
47.45 |
6.21 |
| 16 |
Zertuche, Ana C (11)
|
GUKATY |
|
48.58 |
- |
| 17 |
Larson, Cayleigh B (11)
|
GUKATY |
51.36 |
48.68 |
-2.68 |
| 18 |
Plowman, Alexandra M (11)
|
GUAMBU |
|
50.77 |
- |
| 19 |
Polapally, Anushka N (11)
|
GUKATY |
1:09.31 |
1:01.90 |
-7.41 |
| 20 |
Avery, Kiersten F (11)
|
GUSSAN |
1:08.72 |
1:04.38 |
-4.34 |