| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Hall, Alyssa A (12)
|
GUCFSC |
2:54.40 |
2:44.62 |
-9.78 |
| 2 |
Dao, Alexis C (11)
|
GUCFSC |
2:58.14 |
2:48.18 |
-9.96 |
| 3 |
Zhao, Amelia (12)
|
GUPACK |
2:57.05 |
2:52.56 |
-4.49 |
| 4 |
Curran, Audrey E (12)
|
GUVSST |
3:15.62 |
2:53.36 |
-22.26 |
| 5 |
Orozco, Mariana (11)
|
GUVSST |
3:00.42 |
2:54.74 |
-5.68 |
| 6 |
Dominguez, Isabelle N (12)
|
GUPACK |
3:14.83 |
2:55.57 |
-19.26 |
| 7 |
Bajaj, Ariana (11)
|
GUTWST |
3:08.74 |
3:01.23 |
-7.51 |
| 8 |
Holwerda, Ava S (11)
|
GUTWST |
3:06.64 |
3:01.55 |
-5.09 |
| 9 |
Lopez, Jocelyne (11)
|
GUAMBU |
3:07.75 |
3:06.33 |
-1.42 |
| 10 |
Song, Selina J (12)
|
GUTWST |
3:42.56 |
3:12.34 |
-30.22 |
| 11 |
Battles, Amelia J (11)
|
GUAMBU |
|
3:12.41 |
- |
| 12 |
Richey, Madeline K (11)
|
GUAMBU |
3:28.43 |
3:15.73 |
-12.70 |
| 13 |
Diaz-Oviedo, Andrea I (12)
|
GUHCAP |
3:10.01 |
3:15.91 |
5.90 |
| 14 |
Moreno, Evelyn S (11)
|
GUTEST |
3:32.75 |
3:19.68 |
-13.07 |
| 15 |
Canovas, Mirielle W (12)
|
GUPACK |
3:20.57 |
3:20.04 |
-0.53 |
| 16 |
Luza, Jennifer K (12)
|
GUPACK |
|
3:27.25 |
- |
| 17 |
Hewitt, Julia N (11)
|
GUTEST |
4:00.93 |
3:59.76 |
-1.17 |
|
McCauley, Meghan A (11)
|
GUPACK |
3:34.94 |
DQ |
- |
|
Bhakta, Priyana A (12)
|
GUCFSC |
3:01.43 |
DQ |
- |