| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Guo, Sean Ziyan (8)
|
GURICE |
43.12 |
42.06 |
-1.06 |
| 2 |
Danican, Aidan Y (6)
|
GUESCA |
45.01 |
43.79 |
-1.22 |
| 3 |
Lo, Jordan C (8)
|
GUPEAK |
47.89 |
44.23 |
-3.66 |
| 4 |
Gimenez, Tomas P (8)
|
GULIFE |
51.12 |
45.98 |
-5.14 |
| 5 |
Tamang, Linus R (7)
|
GUPEAK |
45.75 |
48.02 |
2.27 |
| 6 |
Briggs, Jayson R (8)
|
GUCFSC |
46.56 |
48.51 |
1.95 |
| 7 |
Liao, Ethan X (8)
|
GUPEAK |
54.05 |
53.46 |
-0.59 |
| 8 |
Cassani, Emilio N (7)
|
GUCFSC |
1:16.95 |
1:05.52 |
-11.43 |
| 9 |
Selva, Ajay D (6)
|
GUPEAK |
1:42.40 |
1:09.84 |
-32.56 |
| 10 |
Schulz, Daniel K (8)
|
GUCFSC |
1:03.34 |
1:10.70 |
7.36 |
| 11 |
Greenblatt, Russell M (6)
|
GUTASC |
|
1:14.96 |
- |
| 12 |
Jadon, Yashvardhan S (7)
|
GUCFSC |
|
1:31.56 |
- |
|
von Dohlen, Brayden M (7)
|
GUCFSC |
54.15 |
DQ |
- |