| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Binion, Layla K (9)
|
GUAGS |
46.26 |
43.32 |
-2.94 |
| 2 |
Blaine, Ashley J (8)
|
GUPACK |
51.91 |
43.60 |
-8.31 |
| 3 |
Stidham, Brooklyn E (8)
|
GUAMBU |
46.80 |
45.61 |
-1.19 |
| 4 |
Horne, Sarah L (8)
|
GUPACK |
49.06 |
46.19 |
-2.87 |
| 5 |
Slot, Jennifer R (10)
|
GUMARC |
50.31 |
46.36 |
-3.95 |
| 6 |
Mayhle, Valentina M (9)
|
GURICE |
|
47.12 |
- |
| 7 |
Endress, Madelyn H (8)
|
GUKATY |
49.00 |
47.86 |
-1.14 |
| 8 |
Blaine, Allison J (8)
|
GUPACK |
59.91 |
48.98 |
-10.93 |
| 9 |
Oquendo, Jorlaney M (8)
|
GUPACK |
51.27 |
49.50 |
-1.77 |
| 10 |
Yang, Vivian X (8)
|
GUKATY |
52.08 |
50.04 |
-2.04 |
| 11 |
Dierkes, Ella R (8)
|
GUSPA |
1:00.54 |
50.69 |
-9.85 |
| 12 |
Heczey, Bella M (8)
|
GURICE |
|
55.22 |
- |
| 13 |
Leonard, Kylie R (10)
|
GUPACK |
1:04.54 |
58.50 |
-6.04 |
| 14 |
Fu, Emily Y (6)
|
GUKATY |
1:01.66 |
58.76 |
-2.90 |
| 15 |
Vaughn, Areiny J (9)
|
GUPACK |
1:21.21 |
58.93 |
-22.28 |
| 16 |
Zhang, Elizabeth C (6)
|
GUKATY |
1:04.08 |
59.00 |
-5.08 |
| 17 |
Vaughan, Layla L (8)
|
GUPACK |
1:07.61 |
59.96 |
-7.65 |
| 18 |
Diehl, Eleni C (8)
|
GURICE |
|
1:00.40 |
- |
| 19 |
Lopez DE LA Rosa, Luna N (9)
|
GUKATY |
1:01.06 |
1:02.46 |
1.40 |
| 20 |
Akinjaiyeju, Fiayoshola G (7)
|
GUSPA |
|
1:06.83 |
- |
| 21 |
Bonaiti, Grace (10)
|
GUAGS |
|
1:08.94 |
- |