| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Galloway, Cameron E (8)
|
GUSSAN |
44.61 |
41.76 |
-2.85 |
| 2 |
Hsieh, Kaitlyn M (8)
|
GUPACK |
46.07 |
45.96 |
-0.11 |
| 3 |
Wheeler, Delaney R (8)
|
GUCFSC |
46.18 |
46.11 |
-0.07 |
| 4 |
Heredia, Amanda (8)
|
GUCFSC |
47.64 |
46.94 |
-0.70 |
| 5 |
Preddy, Josephine F (8)
|
GUTWST |
45.29 |
47.11 |
1.82 |
| 6 |
Withers, Amelia E (8)
|
GUCFSC |
|
47.41 |
- |
| 7 |
Stidham, Brooklyn E (8)
|
GUAMBU |
49.72 |
47.51 |
-2.21 |
| 8 |
Treadaway, Caeleigh R (8)
|
GUMAC |
48.01 |
48.13 |
0.12 |
| 9 |
Oquendo, Jorlaney M (8)
|
GUPACK |
48.05 |
48.97 |
0.92 |
| 10 |
Wiles, Sydney C (8)
|
GUTWST |
53.05 |
52.66 |
-0.39 |
| 11 |
Mayorca, Francesca A (8)
|
GUCFSC |
|
55.55 |
- |
| 12 |
Craig, Luciana (8)
|
GUDADS |
|
57.29 |
- |
| 13 |
Gutierrez, Isabella J (8)
|
GUCFSC |
1:00.71 |
57.95 |
-2.76 |
| 14 |
Eskinder, Abigail F (8)
|
GUSSAN |
1:04.39 |
1:02.04 |
-2.35 |
| 15 |
Morman, Madelyn A (8)
|
GUCFSC |
1:08.41 |
1:02.68 |
-5.73 |
| 16 |
Albrecht, Emma P (8)
|
GUSSAN |
|
1:03.68 |
- |
| 17 |
Nguyen, Taylor B (8)
|
GUDADS |
|
1:03.95 |
- |
| 18 |
Griffin-Coyle, Addyson G (8)
|
GUMAC |
1:04.89 |
1:07.97 |
3.08 |
| 19 |
Vaughan, Layla L (8)
|
GUPACK |
1:01.89 |
1:08.02 |
6.13 |
| 20 |
Williamson, Violet S (8)
|
GUDADS |
|
1:16.20 |
- |