| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Sosa, Anna M (10)
|
GUMAC |
39.80 |
36.97 |
-2.83 |
| 2 |
Leonard, Kilynn N (10)
|
GUMAC |
38.02 |
37.42 |
-0.60 |
| 3 |
Kleiman, Grace D (10)
|
GUKATY |
39.30 |
38.76 |
-0.54 |
| 4 |
Leonard, Kinley E (9)
|
GUMAC |
41.16 |
38.95 |
-2.21 |
| 5 |
Schueder, Emma J (10)
|
GUSSAN |
40.65 |
41.13 |
0.48 |
| 6 |
Quave, Juliette F (9)
|
GUAGS |
41.68 |
41.76 |
0.08 |
| 7 |
Gallagher, Anabelle G (9)
|
GUMAC |
|
42.29 |
- |
| 8 |
Nguyen, Alanna H (9)
|
GULSST |
43.90 |
43.24 |
-0.66 |
| 9 |
Dickamore, Kathrin I (9)
|
GUSHRK |
39.91 |
43.65 |
3.74 |
| 10 |
Ammons, Natalie G (10)
|
GUPACE |
42.75 |
44.59 |
1.84 |
| 11 |
Patterson, Emma M (10)
|
GUPACK |
49.00 |
45.24 |
-3.76 |
| 12 |
Lopez DE LA Rosa, Luna N (9)
|
GUKATY |
53.99 |
45.32 |
-8.67 |
| 13 |
McGregor, Moira W (9)
|
GUSHRK |
46.00 |
45.78 |
-0.22 |
| 14 |
Duong, Trinity T (9)
|
GUPACK |
|
51.14 |
- |
| 15 |
Blackmon, Kyra L (9)
|
GUAMBU |
49.52 |
51.95 |
2.43 |
| 16 |
Jacobson, Katherine R (9)
|
GUSHRK |
1:01.57 |
59.96 |
-1.61 |