| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Linkous, Caroline H (13)
|
VAHOKI |
5:05.18 |
4:58.03 |
-7.15 |
| 2 |
Shopbell, Ava M (13)
|
VALY |
4:55.22 |
4:59.73 |
4.51 |
| 3 |
Warren, Sarah J (13)
|
VALY |
4:59.32 |
5:06.38 |
7.06 |
| 4 |
Durbin, Kathryn A (14)
|
VAHOKI |
5:18.91 |
5:11.92 |
-6.99 |
| 5 |
Fillo, Caitlin A (13)
|
VAHOKI |
5:41.24 |
5:14.82 |
-26.42 |
| 6 |
Wiersema, Jillian A (13)
|
VAHOKI |
5:34.11 |
5:26.36 |
-7.75 |
| 7 |
Kovarik, Katherine A (14)
|
VALY |
5:38.51 |
5:26.81 |
-11.70 |
| 8 |
Santos, Leah M (13)
|
VAHOKI |
6:13.82 |
5:28.36 |
-45.46 |
| 9 |
Hu, Melissa S (13)
|
VAHOKI |
5:46.71 |
5:34.61 |
-12.10 |
| 10 |
Holt, Ann-Margaret E (13)
|
VALY |
|
5:37.33 |
- |
| 11 |
Clark, Jaelyn A (13)
|
VAHOKI |
5:45.11 |
5:39.49 |
-5.62 |
| 12 |
Cooper, Emma S (13)
|
VALY |
5:39.38 |
5:40.06 |
0.68 |
| 13 |
Sanford, Emily L (13)
|
VAHOKI |
|
5:53.68 |
- |
| 14 |
Gustafson, Zelynn C (13)
|
VALY |
|
6:58.76 |
- |