| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Yu, Sophia (12)
|
VACYAC |
35.58 |
34.78 |
-0.80 |
| 2 |
Ding, Weining (11)
|
VACYAC |
37.23 |
37.38 |
0.15 |
| 3 |
Life, Megan C (12)
|
VACYAC |
41.17 |
40.70 |
-0.47 |
| 4 |
Lawrence, Peyton E (11)
|
VASFY |
42.88 |
40.89 |
-1.99 |
| 5 |
Cramer, Mary e (11)
|
VACYAC |
48.74 |
42.55 |
-6.19 |
| 6 |
Warren, Grace K (12)
|
VALY |
44.86 |
43.18 |
-1.68 |
| 7 |
Cory, Julia E (12)
|
VACYAC |
58.84 |
47.78 |
-11.06 |
| 8 |
Maddox, Karsen A (12)
|
VALY |
48.60 |
48.11 |
-0.49 |
| 9 |
Alimard, Ariana R (11)
|
VACYAC |
54.52 |
50.44 |
-4.08 |
| 10 |
Searwar, Rachel A (11)
|
VALY |
54.76 |
50.69 |
-4.07 |
| 11 |
Cox, Kaylee R (11)
|
VASFY |
56.94 |
50.70 |
-6.24 |
|
D'Errico, Josephine M (11)
|
VACYAC |
|
NS |
- |