| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Staten, Jamil C (14)
|
GUSSAN |
24.46 |
24.37 |
-0.09 |
| 2 |
Gerwick, Gavin P (14)
|
GUSSAN |
25.13 |
25.05 |
-0.08 |
| 3 |
Brooks, Joshua C (13)
|
GUUN-M |
25.37 |
25.53 |
0.16 |
| 4 |
Crenshaw, Maddox L (14)
|
GUUN-S |
26.27 |
25.99 |
-0.28 |
| 5 |
Smith, Peyton A (14)
|
GUSSAN |
26.65 |
26.51 |
-0.14 |
| 6 |
Ivins, Nicholas P (13)
|
GUSSAN |
27.38 |
27.18 |
-0.20 |
| 7 |
Karp, Ygal (14)
|
GUMAC |
27.24 |
27.42 |
0.18 |
| 8 |
Holland, Nolan R (13)
|
GUMAC |
28.11 |
27.48 |
-0.63 |
| 9 |
Hughes, Carson A (13)
|
GUSSAN |
28.46 |
28.00 |
-0.46 |
| 10 |
Lorenz, Boston M (14)
|
GUSSAN |
29.13 |
28.86 |
-0.27 |
| 11 |
Scott, Nathan D (14)
|
GUMAC |
31.22 |
29.03 |
-2.19 |
| 11 |
Otwell, Lane R (13)
|
GUSSAN |
28.20 |
29.03 |
0.83 |
| 13 |
Cress Mandujano, Alecxander (13)
|
GUMARC |
32.07 |
29.04 |
-3.03 |
| 14 |
Ramirez, Bernardo J (14)
|
GUMARC |
30.82 |
29.47 |
-1.35 |
| 15 |
Howse, Michael T (13)
|
GUSSAN |
30.78 |
30.47 |
-0.31 |
| 16 |
Henderson, William S (13)
|
GUSSAN |
|
33.57 |
- |
| 17 |
Kholodnyi, Ilya V (13)
|
GUMARC |
33.85 |
35.10 |
1.25 |
| 18 |
Hollenbeck, Levi G (13)
|
GUMAC |
36.10 |
36.89 |
0.79 |
|
Scott, Connor M (14)
|
GUSSAN |
23.79 |
NS |
- |
|
Lewis, Gabriel A (13)
|
GUMAC |
32.87 |
NS |
- |