| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Satterfield, Nyah (8)
|
GUMAC |
44.43 |
43.61 |
-0.82 |
| 2 |
Rosebush, Allie L (8)
|
GUMAC |
47.73 |
43.88 |
-3.85 |
| 3 |
Rice, Trinity (8)
|
GUAGS |
49.07 |
44.19 |
-4.88 |
| 4 |
Laubsch, Giada G (8)
|
GUMAC |
49.87 |
44.81 |
-5.06 |
| 5 |
Rasmussen, Kelsie E (8)
|
GUAGS |
50.81 |
48.61 |
-2.20 |
| 6 |
Thompson, Ananiah (8)
|
GUAGS |
|
50.16 |
- |
| 7 |
Aragon Ocampo, Luciana (8)
|
GUMAC |
1:01.99 |
51.03 |
-10.96 |
| 8 |
Leonard, Kinley E (8)
|
GUMAC |
55.85 |
52.33 |
-3.52 |
| 9 |
Brown, Emily E (8)
|
GUMARC |
|
54.63 |
- |
| 10 |
Dristas, Emma A (8)
|
GUMAC |
56.84 |
55.09 |
-1.75 |
| 11 |
Skinner, Sarah E (8)
|
GUMAC |
1:07.08 |
59.11 |
-7.97 |
| 12 |
Scheel, Ella J (8)
|
GUMARC |
|
1:02.30 |
- |
|
Christopherson, Reagan L (8)
|
GUMAC |
|
NS |
- |