| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Herbel, Camille C (7)
|
GUSSAN |
22.81 |
23.45 |
0.64 |
| 2 |
Dulworth, Ella M (7)
|
GUSSAN |
23.30 |
24.15 |
0.85 |
| 3 |
Treadaway, Caeleigh R (7)
|
GUMAC |
25.62 |
24.46 |
-1.16 |
| 4 |
VanVossen, Kathryn G (7)
|
GUMARC |
23.43 |
25.49 |
2.06 |
| 5 |
Walker, Elise M (7)
|
GUSSAN |
26.17 |
25.72 |
-0.45 |
| 6 |
Blaylock, Madison L (7)
|
GUMARC |
23.87 |
27.31 |
3.44 |
| 7 |
Griffin-Coyle, Addyson G (7)
|
GUMAC |
27.31 |
27.42 |
0.11 |
| 8 |
Hallmark, Kaylee G (7)
|
GUSSAN |
24.95 |
27.49 |
2.54 |
| 9 |
Hadley, Kaitlyn N (7)
|
GUSSAN |
|
28.15 |
- |
| 10 |
Taylor, Isabelle R (7)
|
GUSSAN |
25.24 |
28.29 |
3.05 |
| 11 |
Coker, Vivienne L (7)
|
GUSSAN |
|
31.42 |
- |
| 12 |
Cosmi, Eliyah J (7)
|
GUMARC |
25.77 |
31.56 |
5.79 |
| 13 |
Metzger, Hadley A (7)
|
GUSSAN |
|
36.85 |
- |
|
Martinez, Fernanda (7)
|
GUMAC |
|
DQ |
- |
|
Armand, Ava G (7)
|
GUSSAN |
24.52 |
NS |
- |