| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Otwell, Jaden N (7)
|
GUSSAN |
37.67 |
37.00 |
-0.67 |
| 2 |
Olson, Amelia E (8)
|
GUCFSC |
47.91 |
43.26 |
-4.65 |
| 3 |
Cavalcanti, Juliana D (8)
|
GUCFSC |
49.22 |
46.44 |
-2.78 |
| 4 |
Avery, Alexis E (8)
|
GUCFSC |
49.82 |
47.52 |
-2.30 |
| 5 |
Pham, Allyson (7)
|
GUCFSC |
45.27 |
47.54 |
2.27 |
| 6 |
Wu, Beibei S (7)
|
GUVSST |
49.18 |
47.86 |
-1.32 |
| 7 |
Chien, Julienne Y (7)
|
GUCFSC |
56.10 |
48.24 |
-7.86 |
| 8 |
Wells, Amelia S (8)
|
GUKATY |
49.96 |
48.38 |
-1.58 |
| 9 |
Hsieh, Kaitlyn M (7)
|
GUCFSC |
1:00.08 |
48.40 |
-11.68 |
| 10 |
Harms, Audrey H (8)
|
GUKATY |
57.21 |
51.07 |
-6.14 |
| 11 |
Fults, Savannah E (8)
|
GUKATY |
58.57 |
53.55 |
-5.02 |
| 12 |
Hynes, Ansley P (8)
|
GUVSST |
|
55.81 |
- |
| 13 |
Brunkhorst, Addison G (8)
|
GUCFSC |
57.25 |
55.95 |
-1.30 |
| 14 |
Herbel, Camille C (7)
|
GUSSAN |
1:05.50 |
56.44 |
-9.06 |
| 15 |
Masch, Bianca D (8)
|
GUKATY |
|
56.58 |
- |
| 16 |
Karymsakov, Adelia (8)
|
GUVSST |
|
59.97 |
- |
| 17 |
Riebel, Natalie G (8)
|
GUCFSC |
1:06.70 |
1:00.80 |
-5.90 |
| 18 |
Wente, Brooklyn E (8)
|
GUCFSC |
56.18 |
1:04.54 |
8.36 |
| 19 |
Hartkemeyer, Brooke C (8)
|
GUCFSC |
|
1:05.60 |
- |
| 20 |
Preddy, Josephine F (7)
|
GUVSST |
|
1:06.14 |
- |
| 21 |
Yao, Yuchen (6)
|
GUVSST |
|
1:07.08 |
- |
| 22 |
Williams, Avaly Y (8)
|
GUVSST |
|
1:10.42 |
- |
|
Shen, Arianna L (7)
|
GUCFSC |
|
DQ |
- |
|
Anda, Carrie (7)
|
GUCFSC |
|
DQ |
- |
|
Yang, Chloe Y (7)
|
GUSSAN |
1:02.79 |
DQ |
- |
|
Marino, Ava G (8)
|
GUSSAN |
55.69 |
NS |
- |