| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Tisdall, Reese H (8)
|
GURICE |
39.71 |
36.73 |
-2.98 |
| 2 |
Steele, Makayla A (8)
|
GUSTA |
38.24 |
38.02 |
-0.22 |
| 3 |
Goktug, Ada (8)
|
GUFRST |
|
39.24 |
- |
| 4 |
McDaniel, Kenley E (8)
|
GUMAC |
39.85 |
42.15 |
2.30 |
| 5 |
Treadaway, Caeleigh R (8)
|
GUMAC |
41.72 |
42.75 |
1.03 |
| 6 |
VanVossen, Kathryn G (8)
|
GUMARC |
42.94 |
44.19 |
1.25 |
| 7 |
Knapp, Sofi (8)
|
GUMAC |
46.66 |
45.47 |
-1.19 |
| 8 |
Heczey, Bella M (8)
|
GURICE |
45.17 |
47.57 |
2.40 |
| 9 |
Griffin-Coyle, Addyson G (8)
|
GUMAC |
45.76 |
47.67 |
1.91 |
| 10 |
Bruce, Olivia L (8)
|
GUMARC |
46.80 |
48.43 |
1.63 |
| 11 |
Scaletta, Carly M (8)
|
GUMAC |
58.40 |
49.54 |
-8.86 |
| 12 |
Brasher, Violet N (8)
|
GUNOCH |
|
50.44 |
- |
| 13 |
Alenneh Ballin, Bre (8)
|
GULIFE |
55.63 |
51.17 |
-4.46 |
| 14 |
Vincent, Grace K (8)
|
GURICE |
|
54.45 |
- |
| 15 |
Barrios, Ana (8)
|
GULIFE |
|
57.84 |
- |
| 16 |
Costesso, Vivian E (8)
|
GUFRST |
|
1:00.85 |
- |
| 17 |
Johnson, Noah (8)
|
GULIFE |
|
1:14.31 |
- |