| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Coulson, Sarah M (12)
|
GUCFSC |
38.23 |
35.14 |
-3.09 |
| 2 |
Waterman, Clare M (12)
|
GUCFSC |
39.97 |
35.35 |
-4.62 |
| 3 |
Paul, Ella K (12)
|
GUCFSC |
35.82 |
35.37 |
-0.45 |
| 4 |
Garcia, Victoria (12)
|
GUUSA |
45.18 |
38.75 |
-6.43 |
| 5 |
Cubillos, Isabella L (12)
|
GUCFSC |
42.43 |
39.98 |
-2.45 |
| 6 |
Espitia, Sara (11)
|
GUVSST |
40.89 |
40.36 |
-0.53 |
| 7 |
Shepherd, Gabriella F (11)
|
GUCFSC |
48.54 |
40.86 |
-7.68 |
| 8 |
Righi, Hannah J (11)
|
GUVSST |
|
41.16 |
- |
| 9 |
Hejl, Taegen D (12)
|
GUUSA |
|
41.50 |
- |
| 10 |
Fawcett, Lillian L (11)
|
GUVSST |
|
43.25 |
- |
| 11 |
Curran, Audrey E (11)
|
GUVSST |
45.75 |
45.31 |
-0.44 |
| 12 |
Lee, Lila A (12)
|
GUUSA |
|
45.33 |
- |
| 13 |
Fielding, Isabelle R (11)
|
GUVSST |
|
45.64 |
- |
| 14 |
Gonzalez, Gabriella R (11)
|
GUCFSC |
50.19 |
48.36 |
-1.83 |
| 15 |
Williams, Holland A (11)
|
GUVSST |
|
50.43 |
- |
| 16 |
Sheridan, Emily M (11)
|
GUUSA |
|
50.97 |
- |
| 17 |
Nureni-Yusuf, Ajibola F (11)
|
GUCFSC |
54.53 |
50.98 |
-3.55 |
| 18 |
Nunez, Erin A (11)
|
GUEPRC |
52.79 |
54.85 |
2.06 |
|
Black, Janeva S (12)
|
GUUSA |
|
DQ |
- |