| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Steele, Makayla A (8)
|
GUSTA |
18.64 |
19.28 |
0.64 |
| 2 |
Goktug, Ada (8)
|
GUFRST |
|
19.45 |
- |
| 3 |
Treadaway, Caeleigh R (8)
|
GUMAC |
23.30 |
21.20 |
-2.10 |
| 4 |
Knapp, Sofi (8)
|
GUMAC |
24.90 |
25.04 |
0.14 |
| 5 |
Alenneh Ballin, Bre (8)
|
GULIFE |
28.70 |
28.16 |
-0.54 |
| 6 |
Scaletta, Carly M (8)
|
GUMAC |
33.80 |
29.83 |
-3.97 |
| 7 |
Griffin-Coyle, Addyson G (8)
|
GUMAC |
30.13 |
29.88 |
-0.25 |
| 8 |
Costesso, Vivian E (8)
|
GUFRST |
30.56 |
33.22 |
2.66 |
| 9 |
Brasher, Violet N (8)
|
GUNOCH |
|
34.05 |
- |
| 10 |
Barrios, Ana (8)
|
GULIFE |
|
34.40 |
- |
|
Vincent, Grace K (8)
|
GURICE |
35.53 |
DQ |
- |