| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Pham, Allyson (8)
|
GUCFSC |
43.69 |
44.64 |
0.95 |
| 2 |
Cabanzo, Arielle V (8)
|
GUESCA |
45.98 |
45.98 |
0.00 |
| 3 |
Stewart, Claire K (8)
|
GUESCA |
49.01 |
50.48 |
1.47 |
| 4 |
D'Anna, Emma L (8)
|
GUCFSC |
54.33 |
52.85 |
-1.48 |
| 5 |
Knapp, Sofi (8)
|
GUMAC |
57.66 |
54.88 |
-2.78 |
| 6 |
Treadaway, Caeleigh R (8)
|
GUMAC |
55.06 |
56.56 |
1.50 |
| 7 |
Heredia, Amanda (8)
|
GUCFSC |
56.27 |
58.39 |
2.12 |
| 7 |
McDaniel, Kenley E (8)
|
GUMAC |
57.65 |
58.39 |
0.74 |
| 9 |
Betts, Ava G (8)
|
GUCFSC |
59.04 |
59.24 |
0.20 |
| 10 |
Figgs, Zoey A (8)
|
GUCFSC |
1:00.25 |
1:00.00 |
-0.25 |
| 11 |
Metzler, Laci M (8)
|
GUESCA |
1:03.09 |
1:01.42 |
-1.67 |
| 12 |
Scaletta, Carly M (8)
|
GUMAC |
|
1:03.99 |
- |
| 13 |
Paul, Lorna E (8)
|
GUCFSC |
1:11.52 |
1:07.89 |
-3.63 |
| 14 |
Cassady, Addison E (8)
|
GUAQUA |
|
1:09.05 |
- |
| 15 |
Rajendran, Tanvi V (8)
|
GUCFSC |
1:05.89 |
1:09.18 |
3.29 |
| 16 |
Parhizi, Andia (8)
|
GULIFE |
|
1:10.60 |
- |
| 17 |
Griffin-Coyle, Addyson G (8)
|
GUMAC |
1:07.43 |
1:10.87 |
3.44 |
| 18 |
Kaddoura, Lana (8)
|
GUESCA |
|
1:31.74 |
- |
|
Adam, Genevieve J (8)
|
GUCFSC |
|
DQ |
- |
|
Tran, Emma N (8)
|
GUCFSC |
1:06.98 |
NS |
- |