| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Coreas, Siumy M (12)
|
GUUSA |
39.47 |
39.74 |
0.27 |
| 2 |
Moozoun, Kianaaz (12)
|
GUVSST |
39.67 |
40.19 |
0.52 |
| 3 |
Waterman, Clare M (12)
|
GUCFSC |
41.65 |
42.48 |
0.83 |
| 4 |
Cooper, Mae K (11)
|
GUVSST |
44.09 |
43.83 |
-0.26 |
| 5 |
Garcia, Victoria (12)
|
GUUSA |
52.68 |
45.07 |
-7.61 |
| 6 |
Cubillos, Isabella L (12)
|
GUCFSC |
45.67 |
45.89 |
0.22 |
| 7 |
Curran, Audrey E (11)
|
GUVSST |
46.54 |
46.60 |
0.06 |
| 8 |
Lee, Lila A (12)
|
GUUSA |
|
47.29 |
- |
| 9 |
Zakielarz, Sonia (11)
|
GUVSST |
47.71 |
47.64 |
-0.07 |
| 10 |
Hejl, Taegen D (12)
|
GUUSA |
|
48.65 |
- |
| 11 |
Righi, Hannah J (11)
|
GUVSST |
1:01.03 |
48.77 |
-12.26 |
| 12 |
Buzzini, Luna G (11)
|
GUVSST |
53.69 |
48.86 |
-4.83 |
| 13 |
Puente, Ava I (12)
|
GUUN-C |
54.16 |
49.15 |
-5.01 |
| 14 |
Roberson, Abigail E (11)
|
GUUSA |
|
50.30 |
- |
| 15 |
Nureni-Yusuf, Ajibola F (11)
|
GUCFSC |
52.20 |
50.33 |
-1.87 |
| 16 |
Sheridan, Emily M (11)
|
GUUSA |
|
51.48 |
- |
| 17 |
Nunez, Erin A (11)
|
GUEPRC |
55.65 |
53.38 |
-2.27 |
| 18 |
Williams, Holland A (11)
|
GUVSST |
|
56.36 |
- |
|
Prabhu, Bethany T (12)
|
GU300 |
|
DQ |
- |
|
Hutchinson, Ainsley J (12)
|
GUKATY |
|
DQ |
- |
|
Metcalf, Emily k (11)
|
GUEPRC |
1:04.21 |
DQ |
- |