| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
McDaniel, Kenley E (8)
|
GUMAC |
43.11 |
39.85 |
-3.26 |
| 2 |
Treadaway, Caeleigh R (8)
|
GUMAC |
47.38 |
41.72 |
-5.66 |
| 3 |
Bolz, Ella R (8)
|
GUMAC |
49.28 |
44.86 |
-4.42 |
| 4 |
Dumitrascu, Sofie (8)
|
GUKATY |
51.91 |
45.37 |
-6.54 |
| 5 |
Knapp, Sofi (8)
|
GUMAC |
46.98 |
46.66 |
-0.32 |
| 6 |
Smyers, Lillie R (8)
|
GUKATY |
46.53 |
47.52 |
0.99 |
| 7 |
Griffin-Coyle, Addyson G (8)
|
GUMAC |
45.76 |
47.58 |
1.82 |
| 8 |
Li, Jessie Y (8)
|
GUKATY |
47.60 |
50.03 |
2.43 |
| 9 |
Coreas, Claire E (8)
|
GUMAC |
51.01 |
53.52 |
2.51 |
| 10 |
Scaletta, Carly M (8)
|
GUMAC |
|
58.40 |
- |
| 11 |
Botens, Phoebe H (8)
|
GUKATY |
51.99 |
1:01.03 |
9.04 |
|
Zhang, Eva J (8)
|
GUKATY |
|
NS |
- |
|
Heidenreich, Emmalee A (8)
|
GUMAC |
1:04.44 |
NS |
- |