| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Maldonado, Ian S (9)
|
GUCFSC |
33.07 |
32.95 |
-0.12 |
| 2 |
Ho, Aidan E (9)
|
GUCFSC |
34.28 |
33.25 |
-1.03 |
| 3 |
Simmons, Tate C (10)
|
GUMAC |
35.03 |
33.30 |
-1.73 |
| 3 |
Kwok, Logan S (9)
|
GUAGS |
36.46 |
33.30 |
-3.16 |
| 5 |
Prabhakar, Prayagh (10)
|
GUCFSC |
33.05 |
33.34 |
0.29 |
| 6 |
Merriken, Wyatt L (10)
|
GUMAC |
33.54 |
33.70 |
0.16 |
| 7 |
Koo, Bailey (10)
|
GUAGS |
33.80 |
34.29 |
0.49 |
| 8 |
Mann, Brayden J (10)
|
GUCFSC |
38.28 |
35.02 |
-3.26 |
| 9 |
Cavazos, Luca A (10)
|
GUCFSC |
33.77 |
36.13 |
2.36 |
| 10 |
Lemp, Charlton G (10)
|
GUCFSC |
36.87 |
36.72 |
-0.15 |
| 11 |
Satterfield, Nicholas (9)
|
GUMAC |
38.53 |
36.90 |
-1.63 |
| 12 |
Yang, Edward (10)
|
GUAGS |
40.51 |
37.97 |
-2.54 |
| 13 |
Biddlecome, Tyler J (9)
|
GUMARC |
46.73 |
41.65 |
-5.08 |
| 14 |
Briggs, Jayson R (9)
|
GUCFSC |
42.16 |
41.71 |
-0.45 |
| 15 |
Paul, Brewer D (10)
|
GUCFSC |
|
41.85 |
- |
| 16 |
Pang, Howard (9)
|
GUAGS |
49.34 |
42.16 |
-7.18 |
| 17 |
McMorris, Daniel P (9)
|
GUCFSC |
47.01 |
43.75 |
-3.26 |
| 18 |
Garcia, Law A (9)
|
GUCFSC |
49.80 |
44.44 |
-5.36 |
| 19 |
Quave, Joseph F (10)
|
GUAGS |
41.62 |
44.65 |
3.03 |
| 20 |
Gonzalez, Alejandro (10)
|
GUMAC |
54.53 |
50.79 |
-3.74 |
| 21 |
Kanatani, Kaden B (9)
|
GUCFSC |
58.18 |
52.08 |
-6.10 |
| 22 |
Allen, Jack E (9)
|
GUCFSC |
55.82 |
52.27 |
-3.55 |
| 23 |
Derrington, Preston T (9)
|
GUCFSC |
57.13 |
52.34 |
-4.79 |
| 24 |
Brewer, Kingston B (9)
|
GUCFSC |
|
54.76 |
- |
| 25 |
Henderson, Landon T (10)
|
GUSSAN |
50.31 |
55.54 |
5.23 |
| 26 |
Chamberlain, Alan A (10)
|
GUCFSC |
|
57.55 |
- |
|
Granda-Moncayo, Santiago (9)
|
GUAGS |
43.91 |
DQ |
- |
|
Shankar, Dhruv S (9)
|
GUCFSC |
|
NS |
- |
|
Ellis, Wesson R (9)
|
GUCATS |
1:15.06 |
NS |
- |