| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Chen, Mei Ning (7)
|
GUVSST |
|
26.87 |
- |
| 2 |
Trevino, Victoria (7)
|
GUCFSC |
|
27.31 |
- |
| 3 |
Espitia, Sofia (8)
|
GUVSST |
34.75 |
27.72 |
-7.03 |
| 4 |
Meermann, Claire A (7)
|
GUUN-V |
51.91 |
28.53 |
-23.38 |
| 5 |
Williams, Avaly Y (8)
|
GUVSST |
|
29.65 |
- |
| 6 |
Preddy, Josephine F (7)
|
GUVSST |
|
29.88 |
- |
| 7 |
Milano, Caroline A (6)
|
GUCFSC |
38.06 |
32.69 |
-5.37 |
| 8 |
Metcalf, Lorelai (8)
|
GUEPRC |
38.34 |
33.09 |
-5.25 |
| 9 |
Yao, Yuchen (5)
|
GUVSST |
|
33.85 |
- |
| 10 |
Pacoma, Kaylee Angela D (6)
|
GUCFSC |
|
33.97 |
- |
| 11 |
Svoboda, Maryanne C (6)
|
GUCFSC |
39.89 |
36.85 |
-3.04 |
| 12 |
Grewal, Jasmine K (6)
|
GUCFSC |
41.88 |
37.72 |
-4.16 |
| 13 |
Zavala, Lauren C (8)
|
GUCFSC |
|
39.15 |
- |
| 14 |
Apukhtina, Katherine (4)
|
GUVSST |
|
51.96 |
- |
|
Anda, Carrie (7)
|
GUCFSC |
47.14 |
DQ |
- |
|
Chen, Yan Ning (5)
|
GUVSST |
|
DQ |
- |
|
Nasr, Isabella Y (6)
|
GUCFSC |
42.09 |
DQ |
- |
|
Maddan, Molly Kate (6)
|
GUCFSC |
|
DQ |
- |
|
Herrera, Sophia G (5)
|
GUCFSC |
|
DQ |
- |
|
Brunkhorst, Addison G (8)
|
GUCFSC |
41.96 |
DQ |
- |
|
Fontenot, Kalyssa (7)
|
GUEPRC |
|
NS |
- |