| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Kwong, Cadence (7)
|
PVNCAP |
25.56 |
24.65 |
-0.91 |
| 2 |
Ismail, Saara F (7)
|
PVMACH |
|
26.60 |
- |
| 3 |
Maffei, Opaline (7)
|
PVNCAP |
29.70 |
28.02 |
-1.68 |
| 4 |
Ghazal, Jordana C (7)
|
PVMACH |
30.51 |
29.54 |
-0.97 |
| 5 |
Casella, Alice C (7)
|
PVNCAP |
30.70 |
30.11 |
-0.59 |
| 6 |
Lawrence, Elizabeth G (7)
|
PVHACC |
|
30.34 |
- |
| 7 |
Chung, Jacqueline Z (7)
|
PVNCAP |
32.60 |
31.05 |
-1.55 |
| 8 |
Weidner, Maya A (7)
|
PVNCAP |
|
31.83 |
- |
| 9 |
Giguere, Madison A (7)
|
PVBWST |
|
32.59 |
- |
| 10 |
Yarmosh, Scarlet J (7)
|
PVMACH |
29.72 |
32.62 |
2.90 |
| 11 |
Brown, Lucy L (7)
|
VATORP |
|
33.95 |
- |
| 12 |
Hall, Emory J (7)
|
VATORP |
|
35.11 |
- |
|
Pierouchakos, Alexandra G (7)
|
PVBWST |
|
DQ |
- |
|
Rey, Layla S (7)
|
VATORP |
40.75 |
DQ |
- |
|
Miller, Nicole E (7)
|
VATORP |
|
DQ |
- |
|
Juarez, Sydney A (7)
|
VATORP |
41.64 |
DQ |
- |
|
Nguyen, Phoebe K (7)
|
PVNCAP |
|
DQ |
- |
|
Reiley, Claire M (7)
|
PVMACH |
|
DQ |
- |
|
Sanchez, Harper O (7)
|
VATORP |
40.32 |
NS |
- |