| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Polifko, Louise C (7)
|
PVNCAP |
26.22 |
24.97 |
-1.25 |
| 2 |
Feng, Sophie F (7)
|
PVNCAP |
25.35 |
25.75 |
0.40 |
| 3 |
Abrams, Sky L (7)
|
PVNCAP |
24.06 |
26.20 |
2.14 |
| 4 |
Walker, Beatrice E (7)
|
PVNCAP |
27.81 |
27.92 |
0.11 |
| 5 |
Lucchese, Alexandra G (7)
|
PVNCAP |
43.02 |
28.33 |
-14.69 |
| 6 |
Benevento, Ava K (7)
|
PVNCAP |
28.87 |
28.53 |
-0.34 |
| 7 |
Orgad, Abigail S (7)
|
PVNCAP |
29.51 |
29.03 |
-0.48 |
| 8 |
Harms, Stella J (6)
|
PVNCAP |
28.78 |
29.65 |
0.87 |
| 9 |
Buchanan, Brooke Taylor (7)
|
PVNCAP |
28.34 |
30.23 |
1.89 |
| 10 |
Muller, Quinn L (7)
|
PVNCAP |
30.43 |
30.76 |
0.33 |
| 11 |
Ter, Anne Valerie V (7)
|
PVNCAP |
|
32.10 |
- |
| 12 |
Naum, Alexandra S (7)
|
PVNCAP |
31.58 |
32.41 |
0.83 |
| 13 |
Thompson, Addison M (7)
|
PVNCAP |
33.09 |
32.67 |
-0.42 |
| 14 |
Eisenhart, Abigail H (7)
|
PVNCAP |
28.93 |
32.90 |
3.97 |
| 15 |
Hirsch, Mikayla B (7)
|
PVNCAP |
31.25 |
35.22 |
3.97 |
| 16 |
Gaston, Aleah S (7)
|
PVNCAP |
25.21 |
35.37 |
10.16 |
| 17 |
Klein, Carolina A (7)
|
PVNCAP |
29.61 |
35.43 |
5.82 |
| 18 |
Hennelly, Makayla N (7)
|
PVNCAP |
49.57 |
37.14 |
-12.43 |
|
Patel, Meera A (6)
|
PVNCAP |
|
DQ |
- |
|
Shive, Keira X (6)
|
PVNCAP |
|
DQ |
- |
|
Sobel, Madeleine J (7)
|
PVNCAP |
33.14 |
NS |
- |