| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
James, Katherine R (6)
|
PVNCAP |
24.81 |
23.92 |
-0.89 |
| 2 |
Shashaty, Elizabeth T (7)
|
PVFXFX |
25.45 |
24.69 |
-0.76 |
| 3 |
Stewart, Linnea S (7)
|
PVNCAP |
26.47 |
25.52 |
-0.95 |
| 4 |
Christensen, Kaitlyn J (7)
|
PVOCCS |
27.09 |
25.83 |
-1.26 |
| 5 |
Martinez, Lara A (7)
|
PVOCCS |
23.64 |
26.04 |
2.40 |
| 6 |
Estes, Lulu F (7)
|
PVOCCS |
25.39 |
26.07 |
0.68 |
| 7 |
Zimmer, Aubrey L (7)
|
PVOCCS |
33.43 |
27.84 |
-5.59 |
| 8 |
Lugiai, Ainsley R (7)
|
PVOCCS |
33.48 |
29.81 |
-3.67 |
| 9 |
Kislowski, Mia B (7)
|
PVOCCS |
36.45 |
30.54 |
-5.91 |
| 10 |
Ortiz, Laila E (6)
|
PVOCCS |
|
30.72 |
- |
| 11 |
Lugiai, Brooke E (7)
|
PVOCCS |
34.05 |
33.20 |
-0.85 |
| 12 |
Tofilon, Sophie J (6)
|
PVTOLL |
32.50 |
39.86 |
7.36 |
|
Nightengale, Cameron G (7)
|
PVOCCS |
|
DQ |
- |
|
Scott, Madeline G (6)
|
PVOCCS |
|
DQ |
- |