| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Roundtree, Charis T (12)
|
PVRIPS |
28.01 |
28.25 |
0.24 |
| 2 |
Edenberg, Haley M (12)
|
PVNCAP |
32.01 |
30.20 |
-1.81 |
| 3 |
Huang, Sophie L (12)
|
PVDRAG |
32.74 |
31.32 |
-1.42 |
| 4 |
Shontz, Isabelle G (12)
|
PVOCCS |
33.34 |
32.21 |
-1.13 |
| 5 |
Caron, Mary A (12)
|
PVOCCS |
35.96 |
33.78 |
-2.18 |
| 6 |
Manko, Genevieve E (12)
|
PVOCCS |
34.21 |
34.66 |
0.45 |
| 7 |
Kelliher, Anna P (12)
|
PVRIPS |
36.26 |
34.78 |
-1.48 |
| 8 |
Miller, Elizabeth M (12)
|
PVOCCS |
40.00 |
38.13 |
-1.87 |
| 9 |
Sweet, Peyton L (12)
|
PVOCCS |
|
42.94 |
- |
| 10 |
Windmiller, Alexia J (12)
|
PVOCCS |
45.78 |
43.79 |
-1.99 |
| 11 |
Tabor, Chloe E (12)
|
PVOCCS |
54.26 |
53.80 |
-0.46 |
| 12 |
Schreibman, Shayna M (12)
|
PVOCCS |
1:00.05 |
55.40 |
-4.65 |
| 13 |
Stanley, Summer Y (12)
|
PVOCCS |
59.78 |
1:05.26 |
5.48 |