| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Bauerlein, John G (9)
|
PVNCAP |
44.59 |
41.59 |
-3.00 |
| 2 |
Stewart, William B (9)
|
PVNCAP |
45.30 |
45.67 |
0.37 |
| 3 |
Radhakrishnan, Neel R (9)
|
PVNCAP |
50.50 |
47.07 |
-3.43 |
| 4 |
Peterson, Davin L (9)
|
PVNCAP |
52.54 |
47.10 |
-5.44 |
| 5 |
Karp, Joel F (9)
|
PVNCAP |
50.19 |
47.90 |
-2.29 |
| 6 |
Hartanto, Adrian (9)
|
PVNCAP |
48.12 |
48.16 |
0.04 |
| 7 |
Silva-Stein, Sacha B (9)
|
PVNCAP |
|
48.52 |
- |
| 8 |
Tejada, Marco O (9)
|
PVNCAP |
51.00 |
48.61 |
-2.39 |
| 9 |
Singh, Siddharth P (9)
|
PVNCAP |
48.56 |
52.08 |
3.52 |
| 10 |
Wong, Alexander E (9)
|
PVNCAP |
56.01 |
52.87 |
-3.14 |
| 11 |
Dew, Brayden A (9)
|
PVNCAP |
51.09 |
53.14 |
2.05 |
| 12 |
Lyden, Conor D (9)
|
PVMACH |
|
55.96 |
- |
| 13 |
Maslowski, Jonathan W (9)
|
PVNCAP |
1:01.49 |
56.32 |
-5.17 |
| 14 |
Yip-Ward, Spencer G (9)
|
PVMACH |
52.52 |
56.55 |
4.03 |
| 15 |
Norcross, Liam A (9)
|
PVNCAP |
1:09.87 |
58.83 |
-11.04 |
| 16 |
McBride, Hudson J (9)
|
PVNCAP |
|
1:00.00 |
- |
| 17 |
Johnson, Carter M (9)
|
PVNCAP |
1:03.98 |
1:00.81 |
-3.17 |
| 18 |
Bumiller, Mason J (9)
|
PVNCAP |
|
1:01.45 |
- |
| 19 |
Asgill, Edmondson A (9)
|
PVNCAP |
1:08.45 |
1:01.96 |
-6.49 |
| 20 |
Norcross, Logan R (9)
|
PVNCAP |
1:02.64 |
1:12.12 |
9.48 |
| 21 |
Gadwale, Nikhil S (9)
|
PVNCAP |
|
1:35.94 |
- |
|
Wood, Ethan W (9)
|
PVNCAP |
|
DQ |
- |