| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Papadopoulos, Frederick A (10)
|
PVYORK |
41.69 |
40.65 |
-1.04 |
| 2 |
Reber, Casen C (9)
|
PVPM |
41.44 |
41.19 |
-0.25 |
| 3 |
Russell, Wyatt D (9)
|
PVYORK |
44.12 |
43.02 |
-1.10 |
| 4 |
Cornell, Andrew D (9)
|
PVPM |
43.82 |
43.48 |
-0.34 |
| 5 |
Park, Jacob (10)
|
PVASA |
43.90 |
43.74 |
-0.16 |
| 6 |
VanZandt, James R (10)
|
PVFBST |
45.21 |
44.67 |
-0.54 |
| 7 |
Costello, Declan L (10)
|
PVASA |
45.35 |
44.74 |
-0.61 |
| 8 |
McGuire, Jeffrey J (9)
|
PVASA |
45.06 |
46.00 |
0.94 |
| 9 |
Badmus, Nathan T (10)
|
PVMSSC |
48.59 |
46.76 |
-1.83 |
| 10 |
Nunes Fuller, Mateus (10)
|
PVMAC |
48.28 |
46.80 |
-1.48 |
| 11 |
Heron, Taran J (9)
|
PVPM |
46.94 |
46.88 |
-0.06 |
| 12 |
Hamamgian, Gabriel K (10)
|
PVPM |
46.00 |
47.56 |
1.56 |
| 13 |
Cocker, Emmett A (9)
|
PVYORK |
50.47 |
49.52 |
-0.95 |
| 14 |
Kim, Thomas H (10)
|
PVPM |
50.58 |
49.72 |
-0.86 |
| 15 |
Fortes, Elijah M (9)
|
PVFBST |
50.66 |
50.92 |
0.26 |