| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Hinrichsen, Tyler A (18)
|
NTLAC |
24.24 |
24.06 |
-0.18 |
| 2 |
Gonzalez, Matthew (16)
|
NTMAC |
23.80 |
24.11 |
0.31 |
| 3 |
Bosuro, Christen M (15)
|
NTMAC |
24.82 |
24.46 |
-0.36 |
| 4 |
January, Michael A (16)
|
NTMAC |
24.24 |
24.52 |
0.28 |
| 5 |
Stauffer, Shea B (16)
|
NTLAC |
24.35 |
24.97 |
0.62 |
| 6 |
Kutch, Noah R (17)
|
NTMAC |
24.40 |
25.33 |
0.93 |
| 7 |
Tadic, Thayne-Robert I (16)
|
NTMAC |
|
25.43 |
- |
| 8 |
Peterson, Jared P (15)
|
NTLAC |
26.00 |
25.49 |
-0.51 |
| 9 |
Cochrane, Carson D (15)
|
NTLAC |
26.14 |
25.58 |
-0.56 |
| 10 |
Choi, Logan S (16)
|
NTLAC |
25.78 |
25.95 |
0.17 |
| 11 |
Stapleton, Jackson L (15)
|
NTMAC |
25.04 |
25.97 |
0.93 |
| 12 |
Baker, Santana D (15)
|
NTLAC |
24.54 |
26.02 |
1.48 |
| 13 |
Cruz, Marco E (15)
|
NTMAC |
29.51 |
28.45 |
-1.06 |