| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Gogineni, Rishi K (14)
|
AMPTSC |
26.28 |
25.66 |
-0.62 |
| 1 |
Frank, Ryan M (13)
|
AMUN |
25.90 |
25.66 |
-0.24 |
| 3 |
Panizzi, Josiah D (14)
|
AMUN |
26.09 |
25.71 |
-0.38 |
| 4 |
Parisi, Michael A (13)
|
AMUN |
27.42 |
25.96 |
-1.46 |
| 5 |
Cheekati, Vibhav A (13)
|
AMSFAC |
27.56 |
27.50 |
-0.06 |
| 6 |
Suzensky, Aiden J (13)
|
AMUN |
27.59 |
27.89 |
0.30 |
| 7 |
Kubistek, Cole J (13)
|
AMNAC |
29.30 |
28.29 |
-1.01 |
| 8 |
Piatek, Aleksander J (13)
|
AMPTSC |
31.50 |
29.50 |
-2.00 |
| 9 |
Frederick, Gavin D (13)
|
AMRACE |
31.26 |
30.08 |
-1.18 |
| 10 |
Rosensteel, Logan W (13)
|
AMUN |
38.48 |
30.67 |
-7.81 |
| 11 |
Motlakunta, Rohit (14)
|
AMSFAC |
31.67 |
31.83 |
0.16 |
| 12 |
Tarbert, Christopher J (13)
|
AMBPR |
32.49 |
31.96 |
-0.53 |
| 13 |
Pietro, Joseph C (13)
|
AMRACE |
36.10 |
34.67 |
-1.43 |
| 14 |
Sundaresh Babu, Riswanth Har (14)
|
AMBPR |
35.70 |
35.65 |
-0.05 |