| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Polifko, Louise C (8)
|
PVNCAP |
46.35 |
47.97 |
1.62 |
| 2 |
Bui, Emma T (8)
|
PVMACH |
51.57 |
48.38 |
-3.19 |
| 3 |
Dressendorfer, Carson E (8)
|
PVMACH |
51.31 |
49.02 |
-2.29 |
| 4 |
Miller, McKenzie L (8)
|
PVMACH |
51.58 |
51.35 |
-0.23 |
| 5 |
Schroeder, Elizabeth C (8)
|
PVNCAP |
56.27 |
53.08 |
-3.19 |
| 6 |
King, Anna M (8)
|
PVMACH |
53.08 |
54.26 |
1.18 |
| 7 |
Ward, Hayden D (8)
|
PVPM |
|
55.44 |
- |
| 8 |
Malinowski, Sophia N (8)
|
PVNCAP |
|
58.20 |
- |
| 9 |
Penetrante, Leia M (8)
|
PVPM |
|
1:00.06 |
- |
| 10 |
Dufault, Audrey A (7)
|
PVNCAP |
1:03.78 |
1:01.36 |
-2.42 |
| 11 |
Demissie, Hannah B (7)
|
PVNCAP |
1:02.70 |
1:02.71 |
0.01 |
| 12 |
Richards, Ella M (7)
|
PVNCAP |
|
1:02.77 |
- |
| 13 |
Park, Audrey Y (8)
|
PVMACH |
1:00.56 |
1:03.38 |
2.82 |
| 14 |
Yoo, Roxanna C (8)
|
PVPM |
1:11.76 |
1:07.84 |
-3.92 |