| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Bui, Emma T (8)
|
PVMACH |
35.83 |
36.97 |
1.14 |
| 2 |
King, Anna M (8)
|
PVMACH |
35.70 |
38.51 |
2.81 |
| 3 |
Dressendorfer, Beatrice L (8)
|
PVMACH |
41.03 |
40.22 |
-0.81 |
| 4 |
Dressendorfer, Carson E (8)
|
PVMACH |
41.93 |
41.05 |
-0.88 |
| 5 |
Wilson, Mara B (7)
|
PVMACH |
41.29 |
42.18 |
0.89 |
| 6 |
Schroeder, Elizabeth C (8)
|
PVNCAP |
44.34 |
42.32 |
-2.02 |
| 7 |
Malinowski, Sophia N (8)
|
PVNCAP |
42.28 |
42.95 |
0.67 |
| 8 |
Dressendorfer, Ellis C (8)
|
PVMACH |
48.16 |
44.13 |
-4.03 |
| 9 |
Penetrante, Leia M (8)
|
PVPM |
43.19 |
44.96 |
1.77 |
| 10 |
Park, Audrey Y (8)
|
PVMACH |
|
45.54 |
- |
| 11 |
Fisher, Elsie M (8)
|
PVPM |
|
46.59 |
- |
| 12 |
Berry, Caroline J (8)
|
PVPM |
49.87 |
46.90 |
-2.97 |
| 13 |
Cota-Robles, Isabella V (8)
|
PVMACH |
43.45 |
48.16 |
4.71 |
| 14 |
Don, Audrey H (8)
|
PVPM |
|
54.87 |
- |
| 15 |
Townsend, Caitlin D (7)
|
PVPM |
1:07.69 |
56.05 |
-11.64 |
| 16 |
Anderson, Emma E (8)
|
PVPM |
59.14 |
56.94 |
-2.20 |
| 17 |
Demissie, Hannah B (7)
|
PVNCAP |
1:04.89 |
59.88 |
-5.01 |
|
Yu, Sophia G (7)
|
PVMACH |
49.86 |
NS |
- |
|
Frantz, Graycen M (7)
|
PVMACH |
58.69 |
NS |
- |