| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Grayson, Kelsey R (12)
|
MRBGNW |
32.10 |
32.25 |
0.15 |
| 2 |
Jones-Torres, Olivia-Ziva J (12)
|
MRPBAC |
32.45 |
32.39 |
-0.06 |
| 3 |
Edelmann, Ansley (12)
|
MRCFJV |
33.05 |
32.83 |
-0.22 |
| 4 |
Zellner-Portela, Natasha N (12)
|
MRNYSA |
34.93 |
33.11 |
-1.82 |
| 5 |
Chaploutskiy, Polina (12)
|
MRPAC |
33.77 |
33.33 |
-0.44 |
| 6 |
Kregel, Marina (12)
|
MRNDAC |
33.50 |
33.38 |
-0.12 |
| 7 |
Boda, Zoe M (12)
|
MRCOND |
34.13 |
33.53 |
-0.60 |
| 8 |
Rubakh, Elise N (12)
|
MRBGNW |
36.03 |
33.81 |
-2.22 |
| 9 |
Kurth, Katie L (12)
|
MRCFJV |
33.90 |
34.11 |
0.21 |
| 10 |
Aikman, Neve C (12)
|
MRYMID |
35.21 |
34.32 |
-0.89 |
| 11 |
Kick, Isabel S (12)
|
MRMWDA |
35.57 |
35.69 |
0.12 |
| 12 |
Rubin, Sally M (12)
|
MRCOND |
35.04 |
36.02 |
0.98 |
|
Oh, Madeline J (12)
|
MRBGNW |
35.21 |
NS |
- |