| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Kuzicki, Kendra J (11)
|
MRYBAR |
40.08 |
39.51 |
-0.57 |
| 2 |
Broge, Amelia H (12)
|
MRWSSC |
44.55 |
43.04 |
-1.51 |
| 3 |
Cummings, Amelia G (11)
|
MRMWDA |
43.36 |
43.38 |
0.02 |
| 4 |
Laffin, Jillian M (11)
|
CTSYS |
45.08 |
43.65 |
-1.43 |
| 5 |
Catenacci, Emma E (12)
|
MRNDAC |
43.73 |
44.37 |
0.64 |
| 6 |
Lawrence, Lily R (11)
|
MRNBS |
46.49 |
46.33 |
-0.16 |
| 7 |
Webb, Sarah A (11)
|
MRNDAC |
44.67 |
46.40 |
1.73 |
| 8 |
Weimer-Jones, Josie F (11)
|
CTSYS |
50.23 |
51.43 |
1.20 |
| 9 |
Moody, Siobhan E (11)
|
MRNBS |
55.83 |
52.58 |
-3.25 |
| 10 |
Mejia, Annabelle L (12)
|
MRNBS |
52.74 |
53.92 |
1.18 |
| 11 |
Muir-Gardner, Laila R (11)
|
MRYBAR |
56.14 |
1:11.99 |
15.85 |