| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Esposito, Grace (10)
|
MRTRI |
33.09 |
32.94 |
-0.15 |
| 2 |
Briceno, Isabella (10)
|
MRNYSA |
34.66 |
33.53 |
-1.13 |
| 3 |
Price, Lily K (10)
|
MRTRI |
35.29 |
34.70 |
-0.59 |
| 4 |
Afanasewicz, Carly M (10)
|
MRNYSA |
37.02 |
36.71 |
-0.31 |
| 5 |
Van Name, Lily (10)
|
MRTRI |
44.66 |
42.73 |
-1.93 |
| 6 |
Kosyakovskiy, Allison (9)
|
MRTRI |
49.14 |
45.39 |
-3.75 |
| 7 |
Lee, Kaitlyn D (9)
|
MRTRI |
47.50 |
47.25 |
-0.25 |
| 8 |
D'Acunto, Bridgette M (9)
|
MRTRI |
56.64 |
47.63 |
-9.01 |
| 9 |
Brown, Sophia F (9)
|
MRTRI |
1:08.90 |
51.01 |
-17.89 |
| 10 |
Johnson, Siel P (9)
|
MRNYSA |
|
51.07 |
- |
| 11 |
Grob, Riley C (9)
|
MRNYSA |
55.57 |
54.85 |
-0.72 |
| 12 |
Mahoney, Kayleigh R (9)
|
MRTRS |
56.66 |
1:00.30 |
3.64 |
| 13 |
Zellner-Portela, Natasha N (10)
|
MRNYSA |
1:05.52 |
1:02.35 |
-3.17 |
|
Roman, Isabella M (9)
|
MRTRS |
1:01.66 |
NS |
- |