| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Brumbaugh, Anna E (12)
|
AMWAAC |
2:58.28 |
2:52.37 |
-5.91 |
| 2 |
Hajnos, Giada K (12)
|
AMINDY |
2:53.83 |
3:02.57 |
8.74 |
| 3 |
Natali, Taylor J (11)
|
AMANSC |
3:12.25 |
3:14.71 |
2.46 |
| 4 |
Keeley, Emma M (11)
|
AMWAAC |
3:32.32 |
3:15.66 |
-16.66 |
| 5 |
Kunkel, Adrianne L (12)
|
AMSTEE |
3:40.23 |
3:16.03 |
-24.20 |
| 6 |
Hollenbeck, Mackenzie E (11)
|
AMANSC |
3:24.50 |
3:16.98 |
-7.52 |
| 7 |
Azzolina, Jovie L (12)
|
AMFAST |
3:34.61 |
3:17.71 |
-16.90 |
| 8 |
Leonard, Alaina L (12)
|
AMWAAC |
3:40.45 |
3:19.40 |
-21.05 |
| 9 |
Murray, Caitlin R (11)
|
AMSTEE |
3:43.69 |
3:19.95 |
-23.74 |
| 10 |
Bell, Savannah (11)
|
AMSHAQ |
3:48.88 |
3:20.91 |
-27.97 |
| 11 |
Empfield, Natalie A (12)
|
AM3RVS |
3:45.99 |
3:33.81 |
-12.18 |
| 12 |
Peterson, Gabriella E (11)
|
AMSTEE |
3:55.55 |
3:47.92 |
-7.63 |
| 13 |
Hatcher, Camille R (12)
|
AMSTEE |
4:00.23 |
4:19.93 |
19.70 |
|
Jubeck, Claire J (11)
|
AMSTEE |
3:29.22 |
NS |
- |