| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Hyun, Noah A (12)
|
MAJW |
32.03 |
32.18 |
0.15 |
| 1 |
Jawork, Jack (11)
|
MAFSA |
40.94 |
40.47 |
-0.47 |
| 2 |
DeSumma, Trevor T (12)
|
MAJW |
32.95 |
33.99 |
1.04 |
| 2 |
Nicholls, Brant C (11)
|
MAJW |
41.79 |
41.01 |
-0.78 |
| 3 |
Park, Brody H (11)
|
MAFSA |
33.08 |
34.97 |
1.89 |
| 3 |
Mainiero, Nicholas (11)
|
MAUN$ |
39.64 |
42.86 |
3.22 |
| 4 |
Yang, Noah S (12)
|
MAJW |
34.79 |
35.01 |
0.22 |
| 4 |
Purumandla, Abhinav R (11)
|
MAJW |
43.88 |
43.83 |
-0.05 |
| 5 |
Trampe, Ronald J (12)
|
MAJW |
35.76 |
36.15 |
0.39 |
| 5 |
Schneider, Tristan C (11)
|
MAJW |
40.68 |
44.62 |
3.94 |
| 6 |
Dobleman, Landon W (12)
|
MAJW |
43.79 |
44.84 |
1.05 |
| 7 |
Slifer, McLaren C (12)
|
MAACAC |
47.88 |
46.71 |
-1.17 |