| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Kenner, Kayla (8)
|
MAEMAC |
49.77 |
42.27 |
-7.50 |
| 2 |
Reppert, Zoe A (8)
|
MAEMAC |
43.47 |
42.54 |
-0.93 |
| 3 |
Tiers, Sophia C (8)
|
MABLUE |
46.99 |
44.17 |
-2.82 |
| 4 |
Watts, Jelena R (8)
|
MRUN-3 |
|
45.61 |
- |
| 5 |
Walborn, Matilda J (8)
|
MAUN-1 |
51.58 |
46.53 |
-5.05 |
| 6 |
Fabiano, Giovanna (8)
|
MAPFY |
|
48.77 |
- |
| 7 |
Hunt, Addison P (8)
|
MABLUE |
57.72 |
49.29 |
-8.43 |
| 8 |
Brunhoeber, Harlow M (8)
|
MAEMAC |
|
49.65 |
- |
| 9 |
Puentes, Meghan E (8)
|
MAEMAC |
|
56.00 |
- |
| 10 |
Wisser, Makenzy H (8)
|
MABLUE |
58.26 |
56.81 |
-1.45 |
| 11 |
Ziemian, Lily C (8)
|
MABLUE |
|
58.40 |
- |
| 12 |
Mallon, Samantha R (8)
|
MABLUE |
|
1:05.11 |
- |
| 13 |
Gower, Madison A (8)
|
MAEMAC |
|
1:10.29 |
- |
|
Nicolosi, Chloe I (8)
|
MABLUE |
|
DQ |
- |
|
Incalcaterra, Charlotte A (8)
|
MABLUE |
|
DQ |
- |
|
Timko, Violet F (8)
|
MABLUE |
|
NS |
- |
|
Rosca, Adina A (8)
|
MAEMAC |
1:01.74 |
NS |
- |