| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Gasgonia, Marina (10)
|
MAPFY |
29.83 |
29.69 |
-0.14 |
| 1 |
Colangelo, Kayanna E (10)
|
MAKIDS |
33.32 |
31.11 |
-2.21 |
| 1 |
Murphy, Ciara J (10)
|
MAKIDS |
39.45 |
36.88 |
-2.57 |
| 2 |
Hickey, Lillian G (9)
|
MATRI |
33.96 |
32.83 |
-1.13 |
| 2 |
Horvat, Isabella E (10)
|
MAUN-T |
39.12 |
37.74 |
-1.38 |
| 3 |
Wolbert, Sydney R (9)
|
MAPFY |
33.25 |
35.16 |
1.91 |
| 3 |
Farag, Hanna A (10)
|
NJHQH |
40.25 |
43.28 |
3.03 |
| 4 |
Bacinski, Riley (10)
|
NJHQH |
34.71 |
36.38 |
1.67 |
| 4 |
Castro, Steffi (10)
|
MABLUE |
42.12 |
43.36 |
1.24 |
| 5 |
Skirlis, Samantha T (10)
|
MABLUE |
47.31 |
44.11 |
-3.20 |
| 6 |
Castro, Emma (10)
|
MABLUE |
42.44 |
45.49 |
3.05 |
| 7 |
Burke, Ella M (10)
|
MABLUE |
47.55 |
46.90 |
-0.65 |
| 8 |
Frascella, Juliana R (9)
|
MATRI |
54.67 |
48.50 |
-6.17 |
| 9 |
Major, Abigail L (9)
|
MABLUE |
52.42 |
50.56 |
-1.86 |
| 10 |
Loebsack, Sydney A (9)
|
MABLUE |
46.57 |
50.57 |
4.00 |
| 11 |
Schellenberger, Lilli E (10)
|
MABLUE |
50.89 |
51.64 |
0.75 |
| 12 |
Simons, Jillian M (10)
|
MABLUE |
50.16 |
52.93 |
2.77 |
| 13 |
Sanders, Ariana P (9)
|
MABLUE |
49.49 |
53.54 |
4.05 |
| 14 |
Felsted, Julia A (10)
|
MABLUE |
1:00.86 |
55.82 |
-5.04 |
| 15 |
Quick, Mikayla A (9)
|
MABLUE |
1:04.41 |
1:02.82 |
-1.59 |