| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Stevenson, Amelia (9)
|
MAUN-5 |
39.47 |
36.75 |
-2.72 |
| 2 |
Gonzales, Lydia R (7)
|
MAUN-2 |
43.55 |
40.39 |
-3.16 |
| 3 |
Kouh, Hailey J (9)
|
MAGSA |
42.34 |
40.84 |
-1.50 |
| 4 |
Lenett, Carly A (9)
|
MAEMAC |
46.01 |
41.94 |
-4.07 |
| 5 |
Sabalaske, Emma C (10)
|
MAGSA |
43.17 |
42.03 |
-1.14 |
| 6 |
Francisco, Emily P (10)
|
MAUPST |
42.92 |
42.47 |
-0.45 |
| 7 |
Dev, Isha (10)
|
MAGSA |
44.92 |
43.47 |
-1.45 |
| 8 |
Cicco, Mackenzie R (9)
|
MAGSA |
44.61 |
43.66 |
-0.95 |
| 9 |
McIvor, Claire S (10)
|
MABEST |
|
44.60 |
- |
| 10 |
Smith, Carley M (10)
|
MAUPST |
44.40 |
44.68 |
0.28 |
| 11 |
Hang, Mia L (10)
|
MAUPST |
50.38 |
46.56 |
-3.82 |
| 12 |
O'Brien, Cecilia M (9)
|
MABEST |
50.46 |
47.21 |
-3.25 |
| 13 |
Emory-Brander, Jenna J (10)
|
MAEAST |
|
49.43 |
- |
| 14 |
Corba, Kameron A (10)
|
MAEMAC |
51.90 |
50.76 |
-1.14 |
| 15 |
Petty, Charlotte A (9)
|
MAGSA |
50.66 |
51.76 |
1.10 |
| 16 |
Alessi, Olivia G (10)
|
MABEST |
|
51.95 |
- |
| 17 |
Shutkufski, Madison H (8)
|
MAEMAC |
|
53.55 |
- |
| 18 |
Doran, Erika L (7)
|
MAUN-2 |
47.88 |
54.82 |
6.94 |
| 19 |
Dutzer, Hanna R (9)
|
MABEST |
|
55.36 |
- |
| 20 |
Talijan, Julianne L (9)
|
MABEST |
|
57.72 |
- |
| 21 |
Jones, Brianna C (9)
|
MAUPST |
56.13 |
58.01 |
1.88 |
| 22 |
Ismail, Salma A (7)
|
MABEST |
|
1:10.74 |
- |
| 23 |
Mohamed, Hana (7)
|
MABEST |
|
1:15.27 |
- |
|
Schmittel, Hailey E (8)
|
MABEST |
|
NS |
- |
|
Rubert, Ashley J (10)
|
MAUPST |
|
NS |
- |