| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Grossman, Isabella W (10)
|
VAUN-D |
38.35 |
38.51 |
0.16 |
| 2 |
Peterson, Mackenzie A (10)
|
VATAC |
38.03 |
39.50 |
1.47 |
| 3 |
Deane, Kacey E (10)
|
VATAC |
42.25 |
40.54 |
-1.71 |
| 4 |
Gahagan, Maggie G (9)
|
VAUN-D |
42.50 |
40.73 |
-1.77 |
| 5 |
Schuurman, Nora V (9)
|
VADIG |
41.36 |
41.20 |
-0.16 |
| 6 |
Pflanz, Lilija M (9)
|
VADIG |
41.51 |
41.67 |
0.16 |
| 7 |
Dixon, Elizabeth A (9)
|
VATAC |
|
43.73 |
- |
| 8 |
Patel, Kayla G (10)
|
VATAC |
|
44.34 |
- |
| 9 |
Morgan, Katrina E (10)
|
VATAC |
44.01 |
47.06 |
3.05 |
| 10 |
Britt, Emily M (10)
|
VATAC |
46.19 |
47.51 |
1.32 |
| 11 |
Lensegrav, Alejandra L (10)
|
VATAC |
50.36 |
48.07 |
-2.29 |
| 12 |
Gage, Hanna A (9)
|
VATAC |
1:00.83 |
54.07 |
-6.76 |