| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Brackenridge, Peyton Q (10)
|
NITWST |
44.54 |
46.19 |
1.65 |
| 2 |
Seelig, Jordan L (10)
|
NIEAST |
47.50 |
49.27 |
1.77 |
| 3 |
Hammond, Evelyn R (9)
|
NITWST |
|
49.49 |
- |
| 4 |
Wierer, Alice L (10)
|
NIHAMB |
50.17 |
49.59 |
-0.58 |
| 5 |
Wagner, Addison (9)
|
NITWST |
53.01 |
49.88 |
-3.13 |
| 6 |
Baker, Catherine M (9)
|
NITWST |
51.09 |
50.86 |
-0.23 |
| 7 |
Tronetti, Coral L (10)
|
NIEMAC |
|
51.13 |
- |
| 8 |
Sellan, Grace I (9)
|
NITTAC |
49.53 |
51.27 |
1.74 |
| 9 |
Dundon, Jordyn M (9)
|
NITTAC |
47.78 |
51.58 |
3.80 |
| 9 |
Spino, Kadence E (9)
|
NITWST |
53.02 |
51.58 |
-1.44 |
| 11 |
Smith, Brooklyn A (9)
|
NIHHSC |
|
51.66 |
- |
| 12 |
Ellis, Kuepper M (10)
|
NIPVSC |
53.29 |
52.28 |
-1.01 |
| 13 |
Lawrence, Ella C (9)
|
NITWST |
51.75 |
56.03 |
4.28 |
| 14 |
Wilson, Sydney N (9)
|
NIBCSR |
|
56.54 |
- |
| 15 |
Kirchberger, Addison M (9)
|
NIHAMB |
58.31 |
56.87 |
-1.44 |
| 16 |
Oakes, Madelyn G (10)
|
NIHHSC |
52.20 |
57.32 |
5.12 |
| 17 |
Ford, Alannah K (10)
|
NITWST |
56.01 |
58.85 |
2.84 |
| 18 |
Reagan, Sarah E (9)
|
NITWST |
|
58.93 |
- |
| 19 |
Martin, Aaliyah C (10)
|
NIBCSR |
|
1:00.43 |
- |
| 20 |
Mescall, Madigan G (10)
|
NITTAC |
59.21 |
1:02.42 |
3.21 |
| 21 |
Hosmer, Sadie M (10)
|
NIPVSC |
57.20 |
1:04.57 |
7.37 |
| 22 |
Zent, Madison L (9)
|
NIHAMB |
1:02.14 |
1:07.93 |
5.79 |
| 23 |
Flewellyn, Elizabeth L (10)
|
NIBCSR |
|
1:08.01 |
- |
|
Bell, Lila R (9)
|
NITWST |
48.46 |
DQ |
- |
|
Rivera, Isabella R (9)
|
NIBCSR |
|
DQ |
- |
|
McIlroy, Amelia M (9)
|
NIHAMB |
1:06.30 |
NS |
- |
|
Green, Tanaj R (9)
|
NIBCSR |
|
NS |
- |