| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Choroser, Penelope T (11)
|
NITWST |
36.88 |
38.06 |
1.18 |
| 2 |
Kleinmann, Isabelle K (11)
|
NIHAMB |
39.62 |
39.28 |
-0.34 |
| 3 |
Johnson, Mara C (11)
|
NITWST |
40.50 |
39.84 |
-0.66 |
| 4 |
Wagner, Ava I (11)
|
NITWST |
44.30 |
42.68 |
-1.62 |
| 5 |
McGill, Rose M (11)
|
NIHAMB |
46.30 |
43.85 |
-2.45 |
| 6 |
Hartung, Ava M (11)
|
NIHHSC |
47.04 |
44.80 |
-2.24 |
| 7 |
Moch, Hope M (11)
|
NITTAC |
43.88 |
47.90 |
4.02 |
| 8 |
Moch, Faith L (11)
|
NITTAC |
52.94 |
55.02 |
2.08 |
| 9 |
Bystrak, Sophia C (11)
|
NITWST |
55.91 |
57.25 |
1.34 |
| 10 |
Bartlett, Emmeline J (11)
|
NITTAC |
54.21 |
57.98 |
3.77 |
| 11 |
Smalls, Amiyah R (11)
|
NIBCSR |
|
1:00.37 |
- |
|
Drews, Jillian J (11)
|
NITWST |
|
DQ |
- |
|
Diehl, Leyna J (11)
|
NITTAC |
49.34 |
DQ |
- |
|
Green, Zaciyah R (11)
|
NIBCSR |
|
NS |
- |