| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Noecker, Sophia E (10)
|
NITWST |
42.74 |
40.28 |
-2.46 |
| 2 |
Daley, Lauren S (9)
|
NITWST |
47.15 |
41.31 |
-5.84 |
| 3 |
Mruzik, Lillian G (10)
|
NIEAST |
44.77 |
41.83 |
-2.94 |
| 4 |
Marshall, Molly E (10)
|
NIEMAC |
56.21 |
47.82 |
-8.39 |
| 5 |
Mersinger, Sophia R (10)
|
NITWST |
|
51.04 |
- |
| 6 |
Walters, Elizabeth J (10)
|
NITWST |
48.08 |
52.90 |
4.82 |
| 7 |
Laverdi, Abigail G (9)
|
NIHAMB |
|
56.73 |
- |
| 8 |
Kerins, Bailey R (10)
|
NIHAMB |
1:05.99 |
57.25 |
-8.74 |
| 9 |
Killelea, Keira m (10)
|
NITTAC |
49.28 |
1:01.16 |
11.88 |
| 10 |
Baker, Chloe R (9)
|
NIEMAC |
58.13 |
1:01.76 |
3.63 |
| 11 |
Kleinmann, Isabelle K (9)
|
NIHAMB |
|
1:05.09 |
- |
| 12 |
Ormond, Grace M (9)
|
NIHAMB |
|
1:12.32 |
- |
|
Canestrari, Willow G (9)
|
NITWST |
|
DQ |
- |
|
Skrzypczyk, Emily A (9)
|
NITWST |
|
DQ |
- |