| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Bellerjeau, Marlowe (8)
|
PVASA |
45.76 |
40.86 |
-4.90 |
| 2 |
Yan, Brianna (8)
|
PVRMSC |
46.85 |
40.98 |
-5.87 |
| 3 |
Ji, KaiLi (8)
|
PVRMSC |
|
43.59 |
- |
| 4 |
Zettler, Julia L (8)
|
PVASA |
|
44.07 |
- |
| 5 |
Yuan, Kaylynn (8)
|
PVRMSC |
|
46.68 |
- |
| 6 |
Harvey, Ellis A (8)
|
PVRMSC |
|
49.26 |
- |
| 7 |
Yang, Chloe M (8)
|
PVRMSC |
52.57 |
50.20 |
-2.37 |
| 8 |
Manning, Isabella S (8)
|
PVRMSC |
|
52.19 |
- |
| 9 |
Lu, Zoe J (8)
|
PVRMSC |
|
52.76 |
- |
| 10 |
Kern, Hannah M (8)
|
PVRMSC |
|
53.98 |
- |
|
Hopkins, Olivia D (8)
|
PVASA |
|
DQ |
- |
|
Katsaros, Charlotte M (8)
|
PVASA |
|
DQ |
- |
|
Perna, Michaela V (8)
|
PVRMSC |
|
DQ |
- |
|
Wu, Lina (8)
|
PVRMSC |
|
DQ |
- |
|
Lee, Zoey (8)
|
PVRMSC |
|
NS |
- |