| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Campbell, Sydney K (7)
|
PVMSSC |
|
44.80 |
- |
| 2 |
Bishop, Alexandra (Lexie) M (7)
|
PVASA |
46.26 |
45.36 |
-0.90 |
| 3 |
Coggeshall, Corinne S (7)
|
PVRMSC |
52.25 |
47.04 |
-5.21 |
| 4 |
Heitzmann, Lily S (7)
|
PVRMSC |
59.29 |
47.28 |
-12.01 |
| 5 |
Yan, Brianna (7)
|
PVRMSC |
|
49.75 |
- |
| 6 |
Brunson, Jada E (7)
|
PVRMSC |
|
50.57 |
- |
| 7 |
Ren, Samantha H (7)
|
PVRMSC |
|
51.61 |
- |
| 8 |
Chen, Zoie (7)
|
PVRMSC |
50.29 |
52.02 |
1.73 |
| 9 |
Tsarni, Kesi R (7)
|
PVRMSC |
|
54.61 |
- |
| 10 |
Wofford, Rayna R (7)
|
PVRMSC |
|
55.96 |
- |
| 11 |
Manning, Isabella S (7)
|
PVRMSC |
|
57.14 |
- |
| 12 |
Yan, Alisia (7)
|
PVRMSC |
|
57.55 |
- |
| 13 |
Lu, Zoe J (7)
|
PVRMSC |
1:01.02 |
57.84 |
-3.18 |
| 14 |
Yuan, Kaylynn (7)
|
PVRMSC |
|
57.92 |
- |
| 15 |
Martin, Amelia B (7)
|
PVASA |
52.79 |
58.13 |
5.34 |
| 16 |
Bascope, Maya M (7)
|
PVMSSC |
1:18.01 |
58.74 |
-19.27 |
| 17 |
Trepkowski, Natalya I (7)
|
PVRMSC |
|
59.69 |
- |
| 18 |
Egwim, Tessa N (7)
|
PVRMSC |
|
59.90 |
- |
| 19 |
Rivera, Bianca G (7)
|
PVRMSC |
|
1:00.86 |
- |
| 20 |
Bracciale, Laila (7)
|
PVRMSC |
|
1:01.12 |
- |
| 21 |
Fiske, Caroline E (7)
|
PVRMSC |
|
1:03.12 |
- |
| 22 |
Harvey, Ellis A (7)
|
PVRMSC |
1:04.57 |
1:03.35 |
-1.22 |
| 23 |
Hernandez Fuentes, Ashley C (7)
|
PVRMSC |
|
1:03.37 |
- |
| 24 |
Lase, Nora I (7)
|
PVRMSC |
|
1:04.25 |
- |
| 25 |
Gopalan, Saisha K (7)
|
PVRMSC |
|
1:04.80 |
- |
| 26 |
Samson, Efratah B (7)
|
PVRMSC |
|
1:21.38 |
- |
| 27 |
Bauer, Katherine J (7)
|
PVRMSC |
|
1:25.84 |
- |
|
Pashkoff, Brodie H (7)
|
PVRMSC |
|
DQ |
- |
|
Lee, Zoey (7)
|
PVRMSC |
1:01.03 |
NS |
- |