| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Goldman, Leah R (20)
|
PCPASA |
1:00.33 |
1:01.32 |
0.99 |
| 2 |
Oglesby, Grace A (17)
|
KYCARD |
1:00.37 |
1:01.58 |
1.21 |
| 3 |
Engel, Ragen E (18)
|
LEFAST |
57.24 |
1:01.99 |
4.75 |
| 4 |
Jorgensen, Whitney E (21)
|
SNSMST |
1:03.50 |
1:02.56 |
-0.94 |
| 5 |
Baxley, Rebecca N (21)
|
PCSRN |
1:03.30 |
1:03.67 |
0.37 |
| 6 |
Anderson, Gabrielle N (16)
|
PCPASA |
1:01.12 |
1:03.78 |
2.66 |
| 7 |
Gaines, Riley M (16)
|
SEXCEL |
1:02.51 |
1:04.19 |
1.68 |
| 8 |
Whisenhunt, Meredith G (19)
|
KYKYA |
1:02.00 |
1:04.39 |
2.39 |
| 9 |
Korenwinder, Mallory A (18)
|
CCTNT |
1:03.28 |
1:05.29 |
2.01 |
| 10 |
Buss, Mackenzie L (21)
|
KYUOFL |
1:05.49 |
1:05.40 |
-0.09 |