| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Shu, Boya V (9)
|
MDEST |
3:02.96 |
3:26.53 |
23.57 |
| 2 |
Pearl, Brooke E (10)
|
MDMAS |
3:04.75 |
3:28.96 |
24.21 |
| 3 |
Casagrande, Claire S (10)
|
MDCAA |
3:04.80 |
3:30.71 |
25.91 |
| 4 |
Piggrem, Scarlett R (10)
|
MDSPY |
3:46.24 |
3:32.32 |
-13.92 |
| 5 |
Ketler, Hannah J (10)
|
MDCAA |
3:45.27 |
3:34.68 |
-10.59 |
| 6 |
Pieninck, Ella G (10)
|
MDEST |
3:21.81 |
3:47.16 |
25.35 |
| 7 |
He, Sophia (9)
|
MDEST |
|
3:49.06 |
- |
| 8 |
Blaha, Adriana N (9)
|
MDEST |
3:40.59 |
3:53.10 |
12.51 |
| 9 |
Wu, Sophie Y (10)
|
MDCAA |
3:22.90 |
3:54.59 |
31.69 |
| 10 |
Cunningham, Isabelle G (10)
|
MDNBAC |
4:00.17 |
3:54.95 |
-5.22 |
| 11 |
Carmer, Madeline A (10)
|
MDRAC |
4:32.17 |
4:01.20 |
-30.97 |
| 12 |
Kenyon, Macall (9)
|
MDCAA |
|
4:04.28 |
- |
| 13 |
Sobotka, Serafina F (10)
|
MDMAS |
3:34.97 |
4:04.34 |
29.37 |
| 14 |
Ryan, Erin A (9)
|
MDEST |
3:38.17 |
4:05.57 |
27.40 |
| 15 |
Feinstein, Samantha M (9)
|
MDEST |
3:45.05 |
4:10.17 |
25.12 |
| 16 |
Temple, Alexandra V (10)
|
MDEST |
3:38.91 |
4:11.88 |
32.97 |
| 17 |
Martinez, Sophia K (10)
|
MDCAA |
|
4:28.14 |
- |
| 18 |
Lucey, Kaylee B (10)
|
MDRAC |
3:49.64 |
4:28.45 |
38.81 |
| 19 |
Liu, Emily F (10)
|
MDCAA |
3:57.26 |
4:30.81 |
33.55 |
| 20 |
Leong, Katrina (9)
|
MDEST |
3:56.39 |
4:32.88 |
36.49 |
| 21 |
Dugas, Ava T (9)
|
MDCAA |
4:05.02 |
4:42.36 |
37.34 |
|
Jung, Ellie E (9)
|
MDEST |
|
DQ |
- |
|
Hopkins, Madison E (9)
|
MDEST |
|
DQ |
- |